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study umuc3  (ATCC)


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    Structured Review

    ATCC study umuc3
    Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from <t>T24</t> CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.
    Study Umuc3, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/study umuc3/product/ATCC
    Average 98 stars, based on 2698 article reviews
    study umuc3 - by Bioz Stars, 2026-05
    98/100 stars

    Images

    1) Product Images from "Alix‐normalized exosomal programmed death‐ligand 1 analysis in urine enables precision monitoring of urothelial cancer"

    Article Title: Alix‐normalized exosomal programmed death‐ligand 1 analysis in urine enables precision monitoring of urothelial cancer

    Journal: Cancer Science

    doi: 10.1111/cas.16106

    Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from T24 CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.
    Figure Legend Snippet: Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from T24 CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.

    Techniques Used: Expressing, Isolation, Microscopy, Transmission Assay, Binding Assay



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    Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from <t>T24</t> CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.
    Study Umuc3, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from <t>T24</t> CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.
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    Image Search Results


    Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from T24 CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.

    Journal: Cancer Science

    Article Title: Alix‐normalized exosomal programmed death‐ligand 1 analysis in urine enables precision monitoring of urothelial cancer

    doi: 10.1111/cas.16106

    Figure Lengend Snippet: Normalization method using exosomal Alix expression. (A) Exosomes (20–200 nm) are secreted from urothelial cancer (UC) and exist in urine in different numbers depending on the condition of the patient. (B) Exosomes were isolated and detected in scanning electron microscope (SEM) and transmission electron microscope (TEM) images. Scale bar, 50 nm. (C) Internal markers such as Alix may have less space for Ab binding compared to surface markers. (D) Intensity analysis of single exosomes for their surface tetraspanin markers (e.g. CD9, CD63, and CD81) and Alix. The intensity of tetraspanin markers (CD9, CD63, and CD81) of particles, isolated from T24 CCS using ExoDisc, attached on the surface coated with CD9, CD63, and CD81 showed varying intensity ranges, whereas Alix showed a uniform intensity range. One‐way AVOVA test was used for statistical analysis. * p < 0.05; ** p < 0.005; **** p < 0.0001; A.U., arbitrary unit; N.S ., not significant; PD‐L1, programmed death‐ligand 1.

    Article Snippet: The cell lines used in this study (UMUC3, T24, RWPE, and A549) were obtained from ATCC.

    Techniques: Expressing, Isolation, Microscopy, Transmission Assay, Binding Assay